| 摘要 |
<p>The invention provides a live gut-colonising microorganism (e.g. Salmonella) capable of evoking an immune response in an animal (e.g. a chicken) for use as a vaccine, characterised in that the microorganism has a specific mutation which impairs its ability to colonise the alimentary tract of the animal. Preferably the microorganism is attenuated, and/or has a negative serological marker, and/or expresses antigens from heterologous organisms. Preferably the mutation comprises the inactivation of a gene selected from: hupA, dksA, rfaY, sipC or clpB. Also disclosed are associated methods generating the vaccines, pharmaceutical preparations, methods of protecting aninals and the products of these methods.</p> |
