| 摘要 |
An automatic focus detection device for microscopes includes an optical system for forming an image of a sample; an image sensor for photoelectrically converting an optical image derived from the optical system; a device for storing image data such that an electric signal obtained from the image sensor is converted from analog to digital form and for processing the image data to compute contrast values; a device for comparing the contrast values; a device for moving an objective lens or a stage for the sample; and a device for storing the position of the objective lens or the stage. In this case, an image contrast at a present position is compared with a contrast at the position where an objective lens or the sample is moved along the optical axis by a predetermined distance, a distance required for movement of the objective lens or the stage is determined from this comparison, and if the distance of movement and a contrast change by the comparison are below constant values, a corresponding position will be decided as the position of the focal plane.
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