Production of 2-keto-L-gulonic acid using pseudogluconobacter saccharoketogenes with recycling

摘要

A method for producing 2-keto-L-gulonic acid (hereinafter referred to as 2KGA) which comprises (1) culturing a microorganism capable of producing 2KGA from L-sorbose, in a liquid culture medium containing a substrate to produce 2KGA and (2) recovering the produced 2KGA, while recovering the microorganism in the culture broth, (3) inoculating the recovered microorganism to a new liquid culture medium for the following culture and (4) repeating said culture and recovering at least once. The present invention provides an industrially advantageous, versatile method for producing 2KGA that shortens the overall culture time, and reduces the amounts of starting medium materials and culture waste.