发明名称 |
Method for monitoring t-cell reactivity |
摘要 |
This invention provides a highly sensitive assay for the detection of T-cells reactive to an antigen by detecting a soluble factor whose secretion is induced by stimulation of the T-cell by the antigen. The assay includes an antigen-driven proliferation of specific T cells prior to restimulation with irradiated antigen presenting cells (APCs) and antigen. The assay can be performed on previously frozen PBMCs, providing greater convenience in sample processing, multiple use of a single sample as an internal standard, and simultaneous analysis of samples collected at different time points. |
申请公布号 |
AU3138797(A) |
申请公布日期 |
1998.01.05 |
申请号 |
AU19970031387 |
申请日期 |
1997.05.20 |
申请人 |
ANERGEN, INC. |
发明人 |
EDWARD G. SPACK;NANCY G. WEHNER;MICHAEL A. MCCUTCHEON |
分类号 |
C12Q1/04;A61K31/00;A61K45/00;A61P37/00;A61P37/06;C12N5/06;G01N33/15;G01N33/50;G01N33/53;G01N33/545;G01N33/564 |
主分类号 |
C12Q1/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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