Purification process for hirudin using affinity chromatography
摘要
Disclosed herein is a process for purifying hirudin from a solution containing hirudin and other substances, characterized in that the process comprises a step of subjecting the solution to a metal ion affinity chromatography wherein copper ion (Cu++) is used as a metal ion and a phosphate buffer is used as an eluent.
申请公布号
US5698104(A)
申请公布日期
1997.12.16
申请号
US19950524311
申请日期
1995.09.06
申请人
DONG KOOK PHARMACEUTICAL CO., LTD.;KOREAN INSTITUTE OF SCIENCE AND TECHNOLOGY
发明人
RHEE, SANG KI;CHUNG, BONG HYUN;CHOI, EUI SUNG;SOHN, JUNG HOON;YOUN, DEOK JOONG;KIM, MYUNG KUK;LEE, HAE DON