PEPTIDE ARRAY AND METHOD

摘要

A method of studying a functional protein comprises providing an array of support-bound oligopeptides with different oligopeptides bound in an immunoreactive state at different locations on the support wherein each oligopeptide has defined amino acid residues at two chosen positions and a mixture of amino acid residues at other positions; applying the functional protein in solution to the array; and identifying support-bound oligopeptides that bind to the functional protein. Secondary oligopeptide libraries are used to further elucidate the amino acid sequence of the target protein. The method is used to elucidate the core residues responsible for the binding of a monoclonal antibody to its antigen beta -factor XIIa.