发明名称 |
METHODS OF DETERMINING ENDOGENOUS THROMBIN POTENTIAL (ETP) AND THROMBIN SUBSTRATES FOR USE IN SAID METHODS |
摘要 |
<p>A method for determining the endogenous thrombin potential of a sample having a total anticoagulant activity of or equivalent to at least 0.07 U ISH/ml, includes using a thrombin substrate or a salt thereof that is soluble in the sample to determine the ETP of the sample. Suitable thrombin substrate include those of the formula P-Val-Xaa-S, in which P is an amino protective group, that is non-aromatic and polar, Val is a valine residue attached via a peptide bond to Xaa, Xaa is an amino acid residue comprising a terminal guanidino group or ureido group separated by at least 2 carbon atoms from the peptide backbond the amino acid residue is attached to S and S is a signal group such as a chromophore that can be enzymatically hydrolyzed. Other substrates include substrates comprising the structure Zaa-Pipecolyl-Yaa-S or Zaa-Pro-Yaa-S, wherein Zaa represents D-Phenylalanine, D-Tryptophan or D-Tyrosine, Pro represents proline, Yaa is an amino acid residue other than arginine and S is a signal marker can also be used. The substrates Boc-Gly-Val-Arg-pNA and H-Glu-Gly-Gly-Val-Arg-pNA are also applicable. Furthermore ETP determination methods can be improved by addition of hydroxylamine to the sample to circumvent defibrination of the sample.</p> |
申请公布号 |
EP0802986(A1) |
申请公布日期 |
1997.10.29 |
申请号 |
EP19960902007 |
申请日期 |
1996.01.10 |
申请人 |
HEMKER, HENDRIK COENRAAD |
发明人 |
HEMKER, HENDRIK, COENRAAD;RIJKERS, DIRK, THOMAS, SIGURD;TESSER, GODEFRIEDUS, IGNATIUS |
分类号 |
A61K38/00;C07K5/06;C07K5/08;C07K5/083;C07K5/087;C07K5/093;C07K5/10;C07K5/113;C07K14/75;C12Q1/56;(IPC1-7):C12Q1/56 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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