| 摘要 |
PURPOSE:To provide the subject enzyme not exhibiting a cyclodextrin- synthesizing ability nor a maltose-decomposing ability, exhibiting a polyphenol glycoside-synthesizing ability and capable of synthesizing the polyphenol glycoside in a high yield from a sugar substrate and a polyphenol acceptor respectively in a wide range as the raw materials. CONSTITUTION:The objective enzyme is obtained by seed culturing a bacterium (Bacillus subtilis, Bacillus thuringiensis, etc.) belonging to Bacillus in an aqueous medium containing a C source such as a soluble starch, an N source such as a polypeptone, inorganic salts and vitamins at pH5 to 9 and at 25 to 50 deg.C according to the shake culture method, subsequently carrying out the main culture by the aeration agitation method and purifying the supernatant, e.g. by filtration of the culture solution. |
