| 摘要 |
The present invention describes homogeneous preparations of a folate receptor-directed metalloprotease (FR-MP), methods for obtaining and using the same. The purified enzyme eluted as a single protein peak on reverse-phase HPLC, and SDS-PAGE revealed a single 63,000 Mr species, which was reduced to 58,000 Mr following deglycosylation. The corresponding gene, antibodies specific for FR-MP and methods for monitoring FR-MP expression are disclosed. |
