发明名称 |
PRODUCTION OF VANILLIN |
摘要 |
A method of producing vanillin comprising the steps of: (1) providing transferulic acid or a salt thereof; and (2) providing trans-ferulate: CoASH ligase activity (enzyme activity I), trans-feruloyl SCoA hydratase activity (enzyme activity II), and 4-hydroxy-3-methoxyphenyl-.beta.-hydroxy-propionyl SCoA (HMPHP SCoA) cleavage activity (enzyme activity III). Conveniently the enzymes are provided by Pseudomonas fluorescens Fe3 or a mutant or derivative thereof. Polypeptides with enzymes activities II and III and polynucleotides encoding said polypeptides. Use of said polypeptides or said polynucleotides in a method for producing vanillin. |
申请公布号 |
CA2250043(A1) |
申请公布日期 |
1997.10.02 |
申请号 |
CA19972250043 |
申请日期 |
1997.03.24 |
申请人 |
INSTITUTE OF FOOD RESEARCH |
发明人 |
RHODES, MICHAEL JOHN CHARLES;WALTON, NICHOLAS JOHN;GASSON, MICHAEL JOHN;NARBAD, ARJAN |
分类号 |
A23L2/00;C12N5/10;C12N9/00;C12N9/88;C12N15/09;C12N15/52;C12N15/82;C12P7/24 |
主分类号 |
A23L2/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|