A method of producing vanillin comprising the steps of: (1) providing trans-ferulic acid or a salt thereof; and (2) providing trans-ferulate: CoASH ligase activity (enzyme activity I), trans-feruloyl SCoA hydratase activity (enzyme activity II), and 4-hydroxy-3-methoxyphenyl- beta -hydroxy-propionyl SCoA (HMPHP SCoA) cleavage activity (enzyme activity III). Conveniently the enzymes are provided by Pseudomonas fluorescens Fe3 or a mutant or derivative thereof. Polypeptides with enzymes activities II and III and polynucleotides encoding said polypeptides. Use of said polypeptides or said polynucleotides in a method for producing vanillin.
申请公布号
WO9735999(A2)
申请公布日期
1997.10.02
申请号
WO1997GB00809
申请日期
1997.03.24
申请人
INSTITUTE OF FOOD RESEARCH;NARBAD, ARJAN;RHODES, MICHAEL, JOHN, CHARLES;GASSON, MICHAEL, JOHN;WALTON, NICHOLAS, JOHN
发明人
NARBAD, ARJAN;RHODES, MICHAEL, JOHN, CHARLES;GASSON, MICHAEL, JOHN;WALTON, NICHOLAS, JOHN