| 摘要 |
<p>The present invention discloses splicing cassettes, vectors, and transformed cells that enable the production of stable introns having antisense sequences. The stable introns are produced during the RNA splicing procedure, wherein RNA lariats are formed having a branch point that cannot be debranched. When introduced into the nucleus of eukaryotic cells, the stable antisense RNAs will inhibit the expression of complementary mRNAs. This inhibitory effect forms the basis of a method of gene therapy that can be used to treat a number of infectious diseases and cancers.</p> |
