METHODS AND MEANS RELATING TO QUIESCENT CELLS AND USES THEREOF

摘要

Rcd expression from an extra-chromosomal vector in bacterial cells in broth culture induces quiescence. Expression continues from extra-chromosomal vectors within the cells during quiescence, while chromosomal expression is suppressed. The cells may be used as factories for the production of large amounts of polypeptides of interest, particularly polypeptides which normally have an adverse effect on cell viability or growth. Expression from an extra-chromosomal vector of interest may be monitored, in view of the reduced background expression from the chromosome. Vector copy number may be amplified. Cell cycles may be synchronised. Suitable cells for rcd-induced quiescence include hns<-> strains of bacteria such as E. coli.