Method of preparing biologically active reagents from succinimide-containing polymers analytical element and methods of use

摘要

Biologically active reagents are prepared from particles of copolymers having highly active succinimide groups. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through amide groups by displacement of highly active succinimide groups on the particle surface. These reagents are used to advantage in analytical elements, methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods such as affinity chromatography.