Biotin-containing compounds, biotinylation reagents and methods

摘要

Water soluble biotin-containing compounds and biotinylation reagents incorporating soluble linker moieties are disclosed. The water soluble biotin-containing compounds and biotinylation reagents may additionally comprise one or more moieties that confers resistance to cleavage by biotinidase or that is cleavable in vitro or in vivo. Biotin-containing compounds and biotinylation reagents may include a reactive moiety that provides a site for reaction with yet another moiety, such as targeting, diagnostic or therapeutic functional moiety. Conjugates including functional moieties are disclosed. Biotin dimers, trimers and multimers comprising water soluble linker moieties which demonstrate enhanced water solubility are also disclosed. Methods for amplifying the number of sites for binding biotin-binding proteins at a selected target using biotin trimer compounds are disclosed. Methods for synthesizing the biotin-containing compounds and biotinylation reagents, and methods for using such compounds are described and an example of the invention, streptavidin cross-linking with biotin derivatives is shown in the figure.