| 摘要 |
<p>PURPOSE:To carry out pH control without adding a buffer agent and damaging cells by removing gaseous carbon dioxide from a liquid of a culture solution, culture filtrate and liquid culture medium to the outside of a membrane and increasing pH of the liquid, introducing the treated liquid into a culture vessel and adjusting the pH. CONSTITUTION:A culture solution in a culture vessel 1 is fed to a cell separator 16 with a pump 6 and separated with a precise filtration membrane 17 to provide a cell-containing fraction, which is returned to the culture vessel. The culture solution is fed into a gaseous carbon dioxide-permeable membrane pipe 3 under pressure with a pump 19. The culture filtrate alkalified by decarbonation is then introduced into an ammonia removing apparatus 20 to remove ammonia with decarbonated air. The alkalified culture filtrate is subsequently returned to the culture vessel 1 with a pump 12 to carry out pH control.</p> |
