| 摘要 |
PURPOSE:To enable the mass production of the title enzyme excellent in heat resistance, mutation resistance and stability by culturing a microorganism in Thermus or Sulfolobus, producing heat-resistant isocitric acid dehydrogenase. CONSTITUTION:For example, a microorganism producing heat-resistant isocitric acid dehydrogenase in Thermus or Sulfolobus, which has been separated from a hot spring is cultured at elevated temperature such as 65-85 deg.C in a usual culture medium for bacteria under neutral for acidic conditions. Then, the product is treated by, e.g., centrifugation or filtration and the fractions rich in the title enzyme is, when necessary, subjected to contaminant proteins, then separated and purified to give the title enzyme of high purity and high activity. |
