DEHYDROGENASE GENE OF POLYVINYL ALCOHOL

摘要

PROBLEM TO BE SOLVED: To obtain the subject gene useful for preparing an expression system capable of decomposing PVA by a single bacterium and mass-producing PVA dehydrogenase as an enzyme preparation. SOLUTION: This PVA dehydrogenase gene codes an amino sequence of the formula (and substantially same amino sequence as the sequence of the formula). Whole DNA extracted from a microorganism having a PVA decomposing enzyme such as Pseudomonas sp. VM15C strain is fragmentated and integrated into a proper vector and a host bacterium such as Escherichia coli is transformed. As a result, a structural gene of PVA dehydrogenase can be cloned. A transformant (pMSVA-46TT) strain by Escherichia coli JM101 is deposited as FERM P-15403.