Method for the production of biological active beta-NGF by genetic engineering

摘要

Production of genetically engineered, biologically active beta -NGF (nerve growth factor) comprises expressing the appropriate DNA in prokaryotic hosts then converting the insoluble aggregates to a soluble active form by: (i) solubilisation; (ii) optionally dialysis and/or derivatisation of beta -NGF with the oxidised form of a compound (A) having thiol groups in reduced form but existing as a disulphide-bridged dimer in oxidised form, so as to produce a mixed disulphide; and (iii) renaturation using a redox system consisting of a compound (B), in oxidised and/or reduced form that is similar to (A); where the process is carried out at 0-30 degrees C and pH 8-10, in presence of Tris base or salt and/or arginine.