| 摘要 |
<p>The invention pertains to increasing the monodispersion of sub-micron emulsions capable of transporting oxygen and intended for use as blood substitutes, radio-opaque agents and agents for preserving isolated organs, and also to improving sterile conditions under which the proposed method of obtaining these emulsions is applied. A high degree of monodispersion is attained by reducing the proportion in the emulsion of large particles greater than 0.2 micron in size. Highly sterile conditions for the production of the emulsions are ensured by carrying out the process in a closed circulation loop of a homogenizer in a single cycle under aseptic conditions. The method is carried out in two closed circulation loops of a homogenizer. The starting emulsion is obtained in the main homogenizer loop by leaking a mixture of perfluoro-organic compounds through an aqueous solution of an emulsifying agent. The emulsion is then passed alternately through the main and auxiliary homogenizer loops, the pressure in the auxiliary circuit being 1.1 to 1.2 times the homogenization pressure in the main circuit. To obtain a 20-40 % emulsion, perfluorodecalin or perfluoroctyl bromide is mixed with perfluoromethylcyclohexyl piperidine in a ratio of 2:1 to 9:1. The emulsifying agent used is a copolymer of polyoxyethylene-polyoxypropylene. The mean particle size is between 0.06 and 0.07 microns. The proportion of particles less than 0.1 micron in size is 85 %.</p> |
