| 摘要 |
PURPOSE: To obtain a pyruvate oxidase for research and diagnosis by incorporating a pyruvate oxidase gene coding a specific amino acid sequence into an expression vector and transfecting a host microorganism with the expression vector to give a transformant and expressing this gene information. CONSTITUTION: A polydeoxyribonucleic acid as a pyruvate oxidase gene coding an amino acid sequence from the N-terminus of the formula (A is an amino acid residue, H, acetyl; B is an amino acid residue, OH, NH2 ) or a polydeoxyribonucleic acid containing this gene as a constitution component is incorporated into an expression vector and a host microorganism is transfected with the recombinant DNA. Then, the transformant is cultured to express the gene information of this polydeoxyribonucleic acid, and a polypeptide as a pyruvate oxidase or a polypeptide including the pyruvate oxidase as a constitution component is collected whereby the objective pyruvate oxidase is efficiently obtained. |
