| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new protein.peptide comprising a thyroid-stimulating hormone which is a new glycoprotein and useful as a diagnostic or a treating medicine for thyroid diseases by partially replacing amino acid of amino acid sequence of a specific protein.peptide. SOLUTION: This new protein.peptide is obtained by replacing Cys<28> with Arg, Met<29> with Arg, Gly<30> with either one of Arg, Ala, Asp, etc., among amino acid of region in an amino acid sequence having protein.peptide of glycoprotein hormone comprising a thyroid-stimulating hormone and represented by the formula and having protein.peptide and useful as a diagnostic or a treating medicine for thyroid diseases. The new protein-peptide is obtained by amplifying a gene coding thyroid.stimulating hormone by PCR method using a synthetic primer comprising a part of the gene, carrying out site-specific mutation of one or more sites among parts corresponding to CyS<28> , Met<29> and Gly<30> of the gene and integrating the gene into a vector and expressing the gene in a host cell. |
