| 摘要 |
<p>Consensus sequences of introns (1, 2 and 3) from the majority of HLA-A, -B and -C allotypes are identified and used to develop primers located within introns (1 and 3) of these genes. The primers are suitable for locus-specific amplification of the entirety of exons (2 and 3). These primers are also suitable for use as sequencing primers to determine the HLA alleles in sequence-based HLA typing. The primers can be used for amplification of portions of introns 1 or 3 and evaluating the amplified products to determine the allelic type of the HLA-A, HLA-B or HLA-C genes. Preferably, one of the primers has a sequence which provides locus-specific amplification.</p> |
