发明名称 |
Transfection and genetic manipulations in obligate intracellular parasites |
摘要 |
A method of transient transfection of obligate intracellular parasites is described. This method is exploited to develop a system for stable transformation utilizing selectable genes. For example, introduction of the chloramphenicol acetyl transferase (CAT) gene fused to Toxoplasma flanking sequences followed by chloramphenicol selection results in parasites stably expressing CAT. DNA hybridization analysis indicated that the CAT gene had inserted via homologous recombination.
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申请公布号 |
US5643718(A) |
申请公布日期 |
1997.07.01 |
申请号 |
US19930147783 |
申请日期 |
1993.11.04 |
申请人 |
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY |
发明人 |
KIM, KAMI;SOLDATI, DOMINIQUE;BOOTHROYD, JOHN C. |
分类号 |
C12N1/11;C12N15/85;C12Q1/68;(IPC1-7):C12Q1/68;C12N15/64;C12P21/02 |
主分类号 |
C12N1/11 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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