| 摘要 |
PROBLEM TO BE SOLVED: To simply and highly sensitively detect a specific DNA by hybridizing the target DNA with a primer, synthesizing a complementary chain containing a fluorescent light label, cleaving the chain, separating the produced oligomers and subsequently detecting the fluorescent light label. SOLUTION: This method for detecting the DNA comprises hybridizing a target DNA 1 with a primer 2 comprising an oligomer, synthesizing a complementary chain 3 containing at least a fluorescent light-labeled deoxynucleotide triphosphate and deoxynucieotldeα-thiotriphosphate as substrates on the primer, cleaving the chain of the DNA produced by the synthesis of the complementary chain by a chemical reaction method or by an enzymatic reaction using an endonuclease, etc., to produce one or more kinds of oligomers 6, separating the produced fragments by a gel electrophoresis method, a liquid chromatograph method, etc., and subsequently measuring the fluorescent light generated from the fluorescent light-labeled fragment 4. Thereby, the specific target DNA can highly sensitively be detected without applying a temperature cycle.
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