A method for expressing recombinant molecules in bacterial hosts in a defined medium in the absence of antibiotic selection is disclosed. The method uses an expression vector comprising a regulatable promoter by which the production of foreign proteins may be controlled during the growth phase of the culture, and origin of replication maintaining medium vector copy number and a transcriptional promoter.
申请公布号
DE69310373(D1)
申请公布日期
1997.06.05
申请号
DE1993610373
申请日期
1993.07.22
申请人
CELLTECH THERAPEUTICS LTD., SLOUGH, BERKSHIRE, GB
发明人
WEIR, ANDREW NEIL CHARLES, TAPLOW, MAIDENHEAD, BERKSHIRE SL6 0NF, GB;MOUNTAIN, ANDREW, BARHAM, WOKINGHAM, BERKSHIRE RG11 4PS, GB