METHODS FOR THE EFFICIENT ISOLATION OF PERIPLASMIC SPACE FRACTIONS FROM MULTIPLE SAMPLES OF BACTERIA

摘要

The invention provides a high throughput device for simultaneously isolating periplasmic fractions from multiple samples of host cells. The device can be formatted to operate in a series. The method includes (a) providing a high throughput device for isolating periplasmic fractions from multiple samples of host cells via attaching a sample chamber to a vacuum chamber and applying a vacuum thereto (b) removing the media with the vacuum and (c) removing the outer membrane of the host cells (d) attaching a collection chamber to the vacuum chamber and applying a vacuum thereto in order to carry out a collection of the periplasmic fractions from the host cells. A workstation is also provided which includes one or more high throughput devices for simultaneously isolating periplasmic space fractions from a plurality of samples of host cells and a device therefore.