| 摘要 |
<p>An assay has been developed to detect the presence of blood clotting factors, including factors VIII and IX, utilizing mutated tissue factor. Mutated forms of wild type tissue factor as well as various forms of truncated tissue factor can be used. Preferably, substitutions of glutamine, alanine, glutamic acid, or combinations thereof are made at amino acid positions 165, 166 or 165 and 166. Various tissue factor mutants exhibit different extents of activity in supporting FVIII and FIX activation: Q?165Q166 > A165A166 > E165E166¿.</p> |
