发明名称 |
Method for counting the number of living microorganisms |
摘要 |
A rapid, convenient, and highly sensitive method for counting the number of living microorganisms is disclosed, wherein a test solution is filtered through a membrane filter element having a plurarity of hydrophilic sections separated from each other by hydrophobic partitions. A microparticulate spray is then applied to the filter in order to add extracting and luminescence-inducing reagents in such an amount that allows the filter membrane within each section to become wet. Further, the sample obtained is processed by applying a highly sensitive luminescence image analyzing system thereto. The employment of a spray method for applying the extracting and luminescence-inducing reagents allows entrapment of the reagents and extracted substances within each of the hydrophilic sections. Therefore, when combined with the use of a luminescence image analyzing system, the number of bright spots so produced of which represent the living microorganisms may be rapidly counted without the requirement of cultivation.
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申请公布号 |
US5627042(A) |
申请公布日期 |
1997.05.06 |
申请号 |
US19950456350 |
申请日期 |
1995.06.01 |
申请人 |
NIHON MILLIPORE KABUSHIKI KAISHA |
发明人 |
HIROSE, DECEASED, ATSUMI;SETO, SUSUMU;KATAOKA, TAKUJI;WANG, DAVID |
分类号 |
C12Q1/06;C12Q1/24;C12Q1/66;(IPC1-7):C02F3/00 |
主分类号 |
C12Q1/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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