Minimierung von Eluatbandbreiten in der Flüssigchromatographie

摘要

In accordance with the disclosed method (300), a surfactant (107) is mixed with a sample (105) prior to injection (303) onto a separation column so that both polar and nonpolar sample components can be enriched at the column head. The components are then separated (304) by reverse-phase gradient elution. The surfactant (107) permits nonpolar analytes to be dissolved in a solvent (e.g., water) which is sufficiently polar such that polar analytes are concentrated along with nonpolar analytes at the head (204) of the column. Thus, on-column enrichment is provided. The effects of the surfactant diminish during elution so that substantial separation of analytes is still obtained. As a result of the on-column enrichment, smaller-bore columns can be used. The smaller-bore columns provide for more concentrated peaks and thus greater detection sensitivity and quantitation accuracy.