Device and method for providing confined reaction and detection

摘要

It is known to carry out PCR amplification and detection using a reaction vessel in which solid particles are fixed and liquid sample and reagents are moved past these particles. Described herein is a device (10, 30) and method for carrying out such reactions. Solids (for example, beads (16)) are used to obtain separation between bound and "free" label reagents, and these are transferred from a first chamber (12) to a second chamber (32), specifically through a wash liquid so as to wash off the "free" label reagent from the solids (16). The first chamber (12) has a cover (14) which is pierced by prong (40) mounted on cover portion (34) of the second chamber (32) to create passageways (36) for the transfer of the solids (16) from the first chamber (12) to the second chamber (32). The passageways (36) remain contained within the device (10, 30) confining the reaction and detection products therein.