| 摘要 |
PROBLEM TO BE SOLVED: To obtain the new subject protein having a specific amino acid sequence, exhibiting the action of an ATP-sensitive potassium channel, useful as a diagnostic medicine and a therapeutic medicine for potassium channel diseases such as diabetes, etc., and further useful for developing the medicines, etc. SOLUTION: This new ATP-sensitive potassium channel protein having an amino acid sequence of the formula and originating from man. The protein is useful as a medicine for diagnosing and treating potassium channel diseases such as diabetes, as a reagent for developing and researching the medicine, etc. The new protein is obtained by screening a human tissue cell-originating genome library or cDNA library with a rat potassium channel whole length cDNA labeled with<32> P, etc., under a standard hybridization condition, subcloning the DNA of the obtained positive clone, determining the base sequence of the subcloned product by a termination method, and subsequently expressing the product in a host cell by a known genetic engineering technology. |
