发明名称 |
Purified gp120 compositions retaining natural conformation |
摘要 |
A method for purifying recombinant HIV gp120 so as to provide a glycopeptide having protein/protein binding properties substantially identical to natural viral HIV gp120, which comprises fractionating a composition containing crude gp120 sequentially using (1) ion exchange chromatography, (2) hydrophobic-interaction chromatography, and (3) size exclusion filtration, collecting at each step a fraction that exhibits specific binding affinity for CD4 peptide. The process is carried out in the absence of any affinity purification steps or any steps (such as reverse-phase HPLC) that use contact protein with organic solvents. The product obtained by this method is a purified, full-length, non-fusion recombinant HIV gp120 glycoprotein having protein/protein-interaction properties substantially identical to gp120 as presented on an HIV virus, including binding affinity for CD4 and binding affinity for at least one antibody capable of neutralizing HIV infectivity.
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申请公布号 |
US5614612(A) |
申请公布日期 |
1997.03.25 |
申请号 |
US19940240073 |
申请日期 |
1994.05.09 |
申请人 |
HAIGWOOD, NANCY L.;SCANDELLA, CARL |
发明人 |
HAIGWOOD, NANCY L.;SCANDELLA, CARL |
分类号 |
A61K39/00;C07K14/16;(IPC1-7):C07K1/00;C07K14/00;A61K39/21 |
主分类号 |
A61K39/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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