ESTERASE-GENE, ESTERASE, REKOMBINANTE PLASMIDE UND DIESE ENTHALTENDE TRANSFORMANTEN, UND VERFAHREN ZUR HERSTELLUNG VON OPTISCH AKTIVER KARBONSÄURE UND IHRER ENANTIOMEREN ESTER DURCH DIESE TRANSFORMANTEN

摘要

The present invention relates to a DNA fragment containing a nucleotide sequence that encodes an amino acid sequence of esterase, said esterase asymmetrically hydrolyzing carboxylic acid esters represented by the formula (I); (wherein R1 is alkyl, aralkyl or aryl, R2 and R3 are alkyl, and n is 1 or 2) an esterase encoded by the DNA fragment, a recombinant plasmid containing the DNA fragment, a microorganism transformed with the recombinant plasmid and methods of producing optically active carboxylic acids and their enantiomeric esters.