CULTIVATION OF ANIMAL CELL

摘要

PROBLEM TO BE SOLVED: To continuously culture an animal cell capable of producing a useful product in high efficiency by using a carrier holding the adhered animal cell, controlling the dissolved oxygen concentration and intermittently exchanging the medium and to easily separate and purify the objective substance in high concentration and efficiency. SOLUTION: This animal cell cultivation method uses a carrier holding adhered animal cells capable of producing a useful product. An expression vector expressing an HGF (hepatocyte growth factor) and integrated with a gene coding for the HGF as the objective substance is introduced into an adhesive animal cell such as CHO cell (Chinese hamster ovarian cell), the transformed animal cell is continuously cultured while controlling the dissolved oxygen concentration to 5-7ppm and intermittently exchanging the medium and the objective substance is separated from the cultured liquid. The accumulation of wastes in the medium is prevented and the objective substance is produced in high concentration and productivity in an easily separable and purifiable state.