RECOMBINANT GAMMA INTERFERON COMPOSITION

摘要

Provided are methods for the production of mixtures of interferon (IFN)- gamma polypeptides. The methods employ a single species of IFN- gamma DNA having codons for Met-Gln and for residues 2-143 of the mature human IFN- gamma amino acid sequence. Expression of such DNA in a suitable recombinant host cell affords a mixture of polypeptides having N-terminal Met-Gln, H-Gln, or H-pyroglutamate residues and C-termini at Arg139, Ser142, or Gln143 of the mature IFN- gamma sequence. The polypeptides so produced exhibit the antiviral and antiproliferative activities as well as the acid lability characteristic of native human IFN- gamma .