HEAT RESISTANT AND GAMMA-CYANO-ALPHA-AMINOBUTYLIC ACID-SYNTHETIC ENZYME AND ITS PRODUCTION

摘要

PROBLEM TO BE SOLVED: To obtain a new synthetic enzyme capable of producing γ-cyano-α- aminobutylic acid from O-acetyl-L-homoserine as a substrate. SOLUTION: This heat resistant and γ-cyano-α-aminobutylic acid-producing enzyme is produced by Bacillus stearothermophilus CN3 strain and has the following properties and actions; the enzyme produces γcyano-α-aminobutylic acid from O-acetyl-L-homoserine and a cyanide compound, the optimum pH: 7.5-8.5, the stable pH range: 6.0-10.5, the optimum temperature: 55-65 deg.C, temperature stability: stable until 60 deg.C when kept at pH 7.5 for 30 minutes, molecular weight: about 180kd by a gel filtration.