发明名称 |
Fluorerscence polarization detection of nucleic acid amplication |
摘要 |
Fluorescence polarization methods for detection of nucleic acid amplification. A fluorescently labelled oligodeoxynucleotide probe is converted from single- to double-stranded form in a target dependent manner during amplification of the target sequence. This conformational change is accompanied by an increase in fluorescence polarization values. The increase in fluorescence polarization can be measured on a transient-state fluorometer in real-time during the amplification reaction without any physical manipulation of the sample. The inventive methods therefore provide a closed, homogeneous system for both amplification of target sequences and detection of amplification. Alternatively, amplification may be detected in the fluorometer after the amplification reaction is completed.
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申请公布号 |
US5593867(A) |
申请公布日期 |
1997.01.14 |
申请号 |
US19940311474 |
申请日期 |
1994.09.23 |
申请人 |
BECTON, DICKINSON AND COMPANY |
发明人 |
WALKER, G. TERRANCE;NADEAU, JAMES G.;LINN, C. PRESTON |
分类号 |
C12N15/09;C12Q1/68;C12R1/32;(IPC1-7):C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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