摘要 |
PURPOSE: To obtain a new Botryococcus braunii B race strain producing hydrocarbons mainly consisting of 30-33C hydrocarbons and containing above a specific weight ratio of a 30C hydrocarbon to the total of hydrocarbons. CONSTITUTION: Planktons on the surface of Ippeki lake (Shizuoka prefecture) are collected by using a Kitahara type plankton net with 25μm mesh. The prepared specimen is observed by a microscope and a colony of an alge of the genus Botryococcus is collected. The colony is cultured in a test tube, a single colony of the alga of the genus Botryococcus is raked by a platinum wire while observing characteristic properties of the alga of the genus Botryococcus in a colony state, etc., by using a stereoscopic microscope and cultured in a float plate medium to give a new Botryococcus braunii B race strain which produces hydrocarbons consisting mainly of 30C to 33C hydrocarbons and containing >=5wt.% 30C hydrocarbon based on the total of hydrocarbons and is Botryococcus braunii SI-1 strain. |