| 摘要 |
The present invention is directed to a computer controlled apparatus for perfusing, cooling, and warming a biological organ. The apparatus includes a programmable computer and a plurality of arterial perfusate reservoirs, each having an input and an output. The contents of the reservoirs are selectively sampled under the control of the computer. An organ container is provided for holding a biological organ. A recirculating fluid flow path connects selected outputs and the inputs of the plurality of reservoirs. An organ flow path extends from the recirculating flow path, through the organ pump, to the organ container. An organ pump is located in said organ flow path and is coupled to and controlled by the computer for withdrawing fluid from the recirculating flow path and delivering the fluid to one or more organs in the organ flow path, thereafter to the recirculating flow path and then either to one of the reservoirs or to the waste outlet, according to control instructions received from the computer. A main pump is interposed in the recirculating flow path and optionally is coupled to and controlled by the computer for circulating perfusate from a selected reservoir through the recirculating fluid flow path, as determined by control instructions received from the computer. Perfusate is selectively returned to a selected one of the reservoirs from the recirculating flow path or to a waste outlet as a function of information sensed by at least one of the sensors. A temperature-controlled cabinet houses the reservoirs, flow paths and pumps to maintain organ and perfusate temperatures at desired levels. A low temperature cooling system is provided for controlling organ temperature over a broad temperature range (as low as -140 DEG C). A low temperature flow path selectively connects the low temperature cooling system to one or both of the flow paths upstream of the organ container. The flow of fluid in the low temperature flow path is controlled also between the cooling system and an outer jacket of the organ container which is used to cool the organ container, and, optionally, to at least one perfusate reservoir. Organ temperature can also be changed by automated immersion means including means capable of cooling organs to below -40 DEG C to -60 DEG C, or warming them from these temperatures. An organ bypass valve allows undesired perfusate to the dead space of the organ flow path to be removed without disconnecting the organ from the perfusate line. |
