Method for anti-viral treatment of biological tissues with collagenic texture

摘要

Antiviral prophylaxis of collagenic tissue, partic. human or animal bone tissue intended for use as biomaterials, comprises eliminating viral contamination by :(i) placing the tissue in a stream of carbon dioxide in the supercritical state; (ii) treating with a hydrogen peroxide soln.; (iii) treating with sodium hydroxide soln.; and (iv) treating with an ethanol soln. Supercritical carbon dioxide is used in an amt. of 100-500 (pref. 300)/g of collagenic tissue at 1-5 (pref. 2.5) x 10<7> Pa at 40-55 (pref. 50) degrees C. Hydrogen peroxide is used in an amt. of at least 1 (pref. 35) wt.% at 30-50 (pref. 40) degrees C. Sodium hydroxide is used in an amt. of 3-15 (pref. 4) wt.%, following which the tissue is rinsed with water and immersed in a neutralising soln. During the final stage of treatment, tissue is immersed in > 90 (pref. 95) vol.% ethanol and then in > 95 (pref. 100) vol.% ethanol