| 摘要 |
<p>Improved porous materials useful in blotting analyses of proteinaceous and genomic matter are prepared by treating porous substrates with a gas plasma containing at least one polymerizable monomer. Thus, a porous sheet-like substrate is modified by means of a deposit of a plasma polymerizate on at least one of its surfaces, whereby this modified surface exhibits reduced nonspecific binding of chemical probes in a blotting analysis. Deposition of the plasma polymer is preferably controlled by means of control of the plasma composite parameter W/FM to less than 1.0 x 10<9> Joules/kilogram. Particularly in the range of 1.0 x 10<8> to 1.0 x 10<9> J/kg for W/FM, nonspecific binding capacity (30) of the modified porous substrate toward oligonucleotide probes is selectively decreased relative to specific binding capacity (31) of the modified porous substrate toward such probes, whereby improved porous materials are obtained having increased signal-to-background ratios and enhanced sensitivities in genomic analyses. In a particularly preferred embodiment, a porous, positively charged nylon membrane is treated with a plasma containing a polymerizable carboxylic acid monomer at a W/FM less than 1.0 x 10<9> J/kg, whereby the membrane surface is modified with an acidic polymeric deposit, this modification resulting in reduced nonspecific binding of oligonucleotide probes and increased sensitivity in chemiluminescent analyses of polynucleotide sequences.</p> |
