发明名称 |
Mammalian/yeast hybrid G protein-coupled receptors |
摘要 |
Methods are disclosed for producing hybrid G protein-coupled receptors. DNA sequences encoding hybrid G protein-coupled receptors are provided, wherein the receptors comprise mammalian G protein-coupled receptors having at least one domain other than the ligand-binding domain replaced with a corresponding domain of a yeast G protein-coupled receptor. DNA constructs comprising the following operatively linked elements: a transcriptional promoter, a DNA sequence encoding a hybrid G protein-coupled receptor, wherein the receptor comprises a mammalian G protein-coupled receptor having at least one domain other than the ligand-binding domain replaced with a corresponding domain of a yeast G protein-coupled receptor, and a transcriptional terminator. Host cells transformed with the DNA constructs and methods utilizing the transformed cells are also provided.
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申请公布号 |
US5576210(A) |
申请公布日期 |
1996.11.19 |
申请号 |
US19940192634 |
申请日期 |
1994.02.07 |
申请人 |
ZYMOGENETICS, INC. |
发明人 |
SLEDZIEWSKI, ANDRZEJ Z.;SHEPPARD, PAUL O. |
分类号 |
C07K14/00;C07K14/37;C07K14/395;C07K14/41;C07K14/705;C07K19/00;C12N1/19;C12N15/09;C12N15/12;C12P21/02;C12R1/85;C12R1/865;(IPC1-7):C12N1/19;C12N15/62 |
主分类号 |
C07K14/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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