PROCESS FOR PRODUCING ERYTHROPOIETIN CONTAINING NO ANIMAL PROTEINS

摘要

A preparation of a protein with erythropoietin activity and obtainable after culturing an erythropoietin-producing host cell is characterised by a) a hostcell proteins content of 100 ppm, b) a host cell DNA content of 10 pg per 83 .mu.g of erythropoietin, and c) a total absence of mammalian proteins not derived from the host cell. The preparation is obtained following serum-free culture using a purification process involving dye chromatography, hydrophobic chromatography on an alkylated or arylated carrier, chromatography on hydroxyapatite, hydrophobic chromatography and anion exchange chromatography.