SERUM-FREE CULTURE MEDIUM AND PRODUCTION OF IN VITRO FERTILIZED EGG

摘要

PURPOSE: To obtain a serum-free culture medium preferably used for in vitro fertilization of fertilized egg and provide a method for producing in vitro fertilized egg. CONSTITUTION: This serum-free culture medium comprises a TCM199 culture medium containing lactic acids, pyruvic acids, basic fibroblast growth factor and tumor growth factor-β1 and having low glucose concentration. The in vitro fertilized egg is produced using the serum-free culture medium. Since the serum- free medium has high generation ratio of fertilized egg and does not contain serum ingredient, stable culture performance is obtained from the serum-free medium. According to the production method, embryo in blastocyst stage used for transplantation of fertilized egg can be generated in high yield from fertilized egg.