发明名称 |
Methods for reducing non-specific priming in DNA detection |
摘要 |
A method for detecting a target nucleic acid, which method includes the steps of (i) amplifying the target nucleic acid to obtain an amplification product using a polymerase, a first primer with or without a segment noncontiguous to a first priming sequence, and a second primer with or without a segment noncontiguous to a second priming sequence in the presence of an oligonucleotide which is incapable of acting as a primer for the polymerase, wherein the oligonucleotide has at least 5 consecutive nucleotides fully complementary to at least 5 consecutive nucleotides of the first primer; and (ii) detecting the presence of the target nucleic acid by monitoring the amplification thereof.
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申请公布号 |
US5567583(A) |
申请公布日期 |
1996.10.22 |
申请号 |
US19940250849 |
申请日期 |
1994.05.26 |
申请人 |
BIOTRONICS CORPORATION |
发明人 |
WANG, CHANG-NING J.;WU, KAI-YUAN |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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