Deubiquitinating enzyme: compositions and methods

摘要

The invention relates generally to compositions of and methods for obtaining deubiquitinating enzyme polypeptides. The invention relates as well to polynucleotides encoding deubiquitinating enzyme polypeptides, the recombinant vectors carrying those sequences, the recombinant host cells including either the sequences or vectors, and recombinant deubiquitinating enzyme polypeptides. By way of example, the invention discloses the cloning and functional expression if at least three different deubiquitinating enzyme polypeptides. The invention includes as well, methods for using the isolated, recombinant enzyme polypeptides in assays designed to select and improve substances capable of interacting with deubiquitinating enzyme polypeptides for use in diagnostic, drug design and therapeutic applications.