发明名称 |
TRANSCRIPTION-BASED NUCLEIC ACID AMPLIFICATION/DETECTION SYSTEM |
摘要 |
A method of amplifying a target sequence in a sample containing nucleic acid is provided which comprises hybridizing to the target sequence a first nucleic acid primer including a promoter sequence, extending the primer in a polymerase extension reaction complementarily to the target sequence to form a duplex, rendering the duplex single stranded, hybridizing to the extension product containing the promoter a second primer and carrying out a second extension reaction, and employing the second duplex as a template for the production of a plurality of RNA transcripts, the method being characterised in that the first and/or second primer includes a sequence necessary to allow the RNA product to be replicated by replicase induction and in that the RNA transcripts are so replicated. |
申请公布号 |
KR960014107(B1) |
申请公布日期 |
1996.10.14 |
申请号 |
KR19890070303 |
申请日期 |
1989.02.19 |
申请人 |
SISKA DIAGNOSTICS INC |
发明人 |
GINGERAS, THOMAS RAYMOND;MERTEN, ULRICH;KWOH, DEBORAH YANTIS |
分类号 |
C12N15/09;C12N15/00;C12N15/10;C12Q1/68;C12Q1/70 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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