PRODUCTION OF ANTIBODIES USING CRE-MEDIATED SITE-SPECIFIC RECOMBINATION

摘要

A method to produce a cell expressing an antibody from a genomic sequence of a cell which has a modified immunoglobulin locus is disclosed. The method takes advantage of Cre-mediated site-specific recombination and homologous recombination. The method involves first introducing a lox site into an immunoglobulin locus and then using the lox site as a target for introduction of gene modifications via Cre-mediated in vivo recombination.