发明名称 |
GENE EXPRESSION IN MAMMALIAN CELLS |
摘要 |
Proteins such as human .beta.-interferon or human erythropoietin are prepared by culturing mammalian cells which harbour a nucleic acid sequence comprising (i) a coding sequence which encodes the desired protein and which is operably linked to a promoter capable of directing expression of the coding sequence in a mammalian cell in the presence of a heavy metal ion; and (ii) a first selectable marker sequence comprises a metallothionein gene and which is operably linked to a promoter capable of directing expression of the metallothionein gene in a mammalian cell in the presence of a heavy metal ion; and optionally (iii) a second selectable marker sequence which comprises a neo gene and which is operably linked to a promoter capable of directing expression of the neo gene in a mammalian cell.
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申请公布号 |
CA2216174(A1) |
申请公布日期 |
1996.10.03 |
申请号 |
CA19962216174 |
申请日期 |
1996.03.22 |
申请人 |
INSTITUTE OF MOLECULAR AND CELL BIOLOGY |
发明人 |
HONG, WANJIN;TAN, YIN HWEE |
分类号 |
C12N15/09;A61K38/00;A61K38/21;A61K38/22;C07K14/505;C07K14/565;C07K14/825;C12N5/10;C12N15/22;C12N15/85;C12P21/02;C12R1/91;(IPC1-7):C12N15/85;C12N15/12;A61K38/18;C12N15/65 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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